Technical Data
DNA Methyltransferase 1 (DNMT, MCMT, M.Hsa1, DNA (cytosine-5-)-methyltransferase 1, DNA MTase Mmu1, M.HsaI)
Inactivation of tumor suppressor genes is a key component of the development of all common forms of human cancers. Aberrant methylation may contribute to many of the changes, both physiological and pathological, associated with aging, including tumor development. Hypermethylation of CpG island-containing promoters is associated with the inactivation of genes important to tumor angiogenesis and progression. DNA methytransferase (DNMT1) is necessary for the maintenance of the methylation process.

Positive Control: T24 cell lysates.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
This antibody was raised against the first 10 kDa of the N-terminus of the DNMT1 protein.
As reported
This antibody is specific for DNMT1. Species Crossreactivity: Recognizes human. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Suzuki, H., et al. A genomic screen for genes upregulated by demethylation and histone deacetylase inhibition in human colorectal cancer. Nature. Doi:10.1038/ng892.
2. Bachman, K., et al. Histone modifications and silencing prior to DNA methylation of a tumor suppressor gene. Cancer Cell. 3:89-95, 2003. (Chromatin Immunoprecipitation, human)
3. Vertino, P., et al. De Novo Methylation of CpG Island Sequences in Human Fibroblasts Overexpressing DNA (Cytosine-5-)- Methyltransferase. Molecular and Cellular Biology.16(8):4555-4565, 1996. (Western blot, Immunohistochemistry, human)
4. Neumeister, P., et al. Senescence and epigenetic dysregulation in cancer. Int. J. Biochem. & Cell Biol. 34:1475-1490, 2002.
5. Yang, X., et al. Collaborative spirit of histone deacetylases in regulating chromatin structure and gene expression. Current Opinion in Genetics & Development. 13:1-11, 2003.