Technical Data
DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1, HSPF1)
DNAJ is a prokaryotic heat shock induced protein from the bacterium E.coli, which is under control of the htpr regulatory protein (1). DNAJ interacts with the chaperone Hsp70-like DNAK protein to disassemble a protein complex found at a phage lambdas origin of replication (ORI). DNAJ is also involved in a variety of difference biological processes in a living cell, including
protein folding and transport, cell survival at high temperatures, negative autoregulation of the heat shock response, and the replication of bacteriophage lambda and other plasmids (2, 3).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot:1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
200ul-20CBlue IceChlamydomonas reinhardtiiRabbit
Not determined.
Recombinant DnaJ of Chlamydomonas reinhardtii.
Supplied as a lyophilized powder. Reconstitute with 200ul of sterile water.
Recognizes Chlamydomonas reinhardtii DnaJ.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gohre, et al. (2006) The Plant Cell. 18: 1454-1466. 2. Pellecchia M, Szyperski T., Wall D., Georgopoulos C., and Wuthrich K. (1996) J Mol Biol. 260: 236. 3. Szyperski T., Pellechia M., Wall D., Georgopolous C., and Wuthrich K. (1994) Proc Nat Acad Sci USA. 91:11343.