		Technical Data
D6500-10B	DHPR, alpha 2 (Dihydropyridine Receptor)

Description:
Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.

Applications:
Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500
Immunofluorescence: 1:500
Immunohistochemistry (Frozen, paraffin): 1:500
Immunoprecipitation: 1:500
Immunocytochemistry: 1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	8J1298	Ascites

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Rabbit	Mouse

Concentration:

Not Determined

Immunogen:

Purified rabbit dihydropyridine receptor.

Purity:

Ascites

Form

Supplied as a liquid, 0.05% sodium azide.

Specificity:

Recognizes 1,4-dihydropyridine (DHP) receptor alpha-2 subunit from rabbit skeletal and cardiac muscle. Detects a band at ~220kD under non-reducing and a ~143kD band under reducing conditions representing DHP from rabbit skeletal muscle membrane preparations. IHC staining of DHP in rabbit skeletal muscle results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines. Species Crossreactivity: human, rat, mouse and guinea pig.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Morton, M.E. & Froehner, S.C., Neuron 2: 1499-1506 (1989). 2. Protasi, F., et al., J. Cell Biol. 140: 831-842 (1998). 3. Flucher, B.E., et al., Neuron 5: 339-351 (1990).