Technical Data
D8060-11
Dinitrophenol (DNP)
Description:
Asthma is a chronic lung disease characterized by airway hyper-responsiveness (AHR) to allergens, airway edema, and increased mucus secretion. Increased levels of circulating IgE and IgG1 antibody and a propensity to allergic responses, atopy, are associated with the development of asthma. Animal models of AHR, where control of the timing of exposure to the initiating antigen, the use of a defined allergen trigger, and genetic manipulation are likely to enhance the understanding of AHR. Dinitrophenol (DNP) has long been employed as a model immunogen as a single antigen to reduce the complexity of modeling AHR.
One of the primary aspects of the immune response is the interaction of antigens with lymphocytes to induce the formation of antibodies, that in turn makes the antigen harmless. Much of our current understanding of the antibody response to antigens has been derived by using the antibody-hapten model. One known model uses the dinitrophenyl (DNP) group. Immunization of many mammalian species with DNP-protein conjugates results in production of antibodies specific for DNP and the amino acid side chains to which it is attached.
DNP conjugates (DNP-KLH or DNP-BSA) useful for the production of antibodies specific for DNP and hemocyanin or BSA. DNP-immunization produced a significant variation in the amount and antibody class (IgGs, IgA, IgE, IgM) among strains, and under various experimental conditions. DNP preparations (purity and supplier), doses (amount per injection), routes (intramuscular, intravenous, aerosol, liposome entrapped, polymerized etc), frequency of exposure (single injections, multiple etc) may induce a defined class of antibody and its level may vary as well. DNP-induced antibody production has been used to assess the immune status of normal and immune-compromised animals.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k7C26Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRat
Concentration:
As reported
Immunogen:
DNP-KLH
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes Dinitrophenol.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.