		Technical Data
D8080-02C	Dok2, p56 (Docking Protein, Downstream of Tyrosine Kinase, Dok-R, p56Dok2, FRIP, rasGAP-associated docking protein)

Description:
The binding protein Dok-2 is also known as p56dok-2. It is a 56kD tyrosine phosphorylated protein, purified and cloned from p210bcr-abl expressing cells, is able to bind to p120RasGAP. There is 35% identity to Dok-1 (p62dok-1). Dok-2 is a rasGAP-associated docking protein expressed mostly in hematopoietic cells. Dok-2 is phosphorylated upon activation of many protein tyrosine kinases (PTKs), including those coupled with cytokine receptors and oncogenic PTKs like Bcr-Abl. Loss of Dok-1 and/or Dok-2 in a knock out mice model carrying the bcr-abl gene resulted in progression of a myeloproliferative disease resembling human chronic myelogenous leukemia. The mouse model suggests that both Dok-1 and Dok-2 suppress leukemia as negative regulators of cytokines responses as well as maintain myeloid homeostasis.

Applications:
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000. Detects ~60kD band.
Immunocytochemistry:1:300
ELISA: 1:100,000 (peptide).

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to aa393-412 with added cysteine (C-ASGWQPGTEYDNVVLKKGPK) from the C-terminus of human p56dok2.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide before the addition of 40% glycerol.

Specificity:

Recognizes human p56dok2 (~55-60kD). Species Crossreactivity: May crossreact with mouse p56dok2 RasGAP-binding protein.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Yasuda, T., Shirakata, M., Iwama, A., Ishii, A., Ebihara, Y., Osawa, M., Honda, K., Shinohara, H., Sudo, K., Tsuji, K., Nakauchi, H., Iwakura, Y., Hirai, H., Oda, H., Yamamoto, T., Yamanashi, Y.: Role of Dok-1 and Dok-2 in myeloid homeostasis and suppression of leukemia. J. Exp. Med. 200(12): 1681-7 (2004). 2. Di Cristofano, A., et al.: Molecular cloning and characterization of p56dok-2 defines a new family of RasGAP-binding proteins. J. Biol. Chem. 273(9): 4827-4830 (1998).