Technical Data
D9000-13
Dopamine Receptor, D2s, aa239-246
Description:
The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. 2 isoforms are reported for the D2 Dopamine Receptor, Isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 40ug/ml using whole rat brain homogenate resulted in a single band being detected at ~48kD.
Immunohistochemistry/Immunocytochemistry: 80ug/ml stains specific cells in various regions of PLP fixed rat brain sections including the Medial septum, Nuclcus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra teticulata and the Ventral regmental area.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
D2s(AC-239-246-Cys247) covalently attached onto a carrier protein.
Purity:
Purified by precipitation, dialysis and chromatography.
Form
Supplied as a lyophilized powder from 100mM ammonuim bicarbonate. Reconstitute with 100ul sterile PBS, 10mg/ml BSA.
Specificity:
Recognizes Dopamine Receptor, D2s, aa239-246. Species Crossreactivity: rat. Crossreacts with Dopamine Receptor, D2s (50%). Does not react with D2L Dopamine Receptor, D1 Dopamine Receptor (9-21), D1 Dopamine Receptor, D3 Dopamine Receptor (2-10), D3 Dopamine Receptor, D4 Dopamine Receptor (176-185), D4 Dopamine Receptor, D5 Dopamine Receptor (23-35), D5 Dopamine Receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.