Technical Data
D9017-20
Dopamine Receptor, D3, D3R
Description:
Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). The two isoforms of D2R, D2 long (D2L) and short D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29 AA in the intracellular domain 3 of the D2 long form. It may play a role in the coupling of the receptor to G- proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell or tissue may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml. Using ECL
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry: 2-10ug/ml
Optimal dilutions to be determined by the researcher.

Control Peptide:
D9017-22: Dopamine Receptor, D3, D3R, Human

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide sequence of 19aa from the human D3 receptor within the 3rd cytoplasmic domain (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity:
Recognizes human Dopamine Receptor, D3. No significant sequence homology with other dopamine receptors (D1, D2, D4 or D5). Species sequence homology: monkey, 94%; mouse, 72%; rat, 68%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Giros B et al (1990) CR Acad. Sci. Vie 311, 501-508; Schmauss C et al (1993) PNAS 90, 8942-8946; Liu K et al (1994) JBC 269, 29220-29226.
2. Fu D et al (1995) DNA Cell Biol 14, 485-192; Sokoloff P et al (1990) Nature 347, 146-151; Giros B et al (1991) BBRC 176, 1584-1592; Livingstone CD et al (1992) Bichem J 287, 277-282.
Citations:
Cho D-I 2003, Life Sciences, 73, 2991-300, WB, IHC, IP, Chu, Eugenia, 2000, JPET 293: 710-716, IHC, Zeng C, 2002, Hypertension, 41, 724-729, WB, IP, Zeng C, 2004, Hypertension, 43: 673 679, WB, IHC, IP, Zeng C 2004, Hypertension, 43: 654 - 660 WB, IP, Ladines, CA, 2001, AJP Regul. Integ. Comp Physiol 281, R1071