		Technical Data
D9641-01D	DVL3 (Dishevelled-3, Dsh Homolog 3, Segment Polarity Protein Dishevelled Homolog DVL-3, KIAA0208)

Description:
Dishevelled-3 (DVL-3) is a cytoplasmic phoshoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes including segmentation and neuroblast specification in Drosophila. It is known to play a crucial role in WNT canonical signaling pathway and has been shown to be a connecting link between the Notch-WNT crosstalk. The structural and evolutionary characteristics suggest that mutations at their loci may be involved in neural and heart development defects.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa560-610 of human DVL-3.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

Specificity:

Recognizes human DVL-3. Species Crossreactivity: chimpanzee, canine, equine, monkey, mouse, rat, rhesus monkey. Species sequence homology: chicken.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Klingensmith, J. et al. Genes Dev. 8:118-130 (1994). 2. Sun, T.Q. et al. Nat. Cell Biol. 3:628-636 (2001). 3. Pizzuti, A. et al. Hum. Mol. Genet. 5:953-958 (1996).