Technical Data
Media
Dulbecco’s MEM (DMEM) F-12, Reh Modification w/o L-Lysine HCl, L-Glutamine, L-Methionine, L-Aspartic Acid, L-Glutamic Acid, Phenol Red, Low Sodium Chloride
D9807-05B
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2O 0.154
Cupric Sulfate•H2O 0.0000013
Ferric Nitrate•9H2O 0.00005
Ferrous Sulfate•7H2O 0.000417
Magnesium Chloride•6H2O 0.0612
Magnesium Sulfate 0.04884
Potassium Chloride 0.3118
Sodium Chloride 4.09
Sodium Phosphate dibasic 0.07102
Sodium Phosphate monobasic 0.0625
Zinc Sulfate•7H2O 0.000432
Amino Acids:
L-Alanine 0.00445
L-Arginine•HCl 0.1475
L-Asparagine•H2O 0.0075
L-Aspartic Acid Absent
L-Cysteine•HCl•H2O 0.01756
L-Cystine•2HCl 0.03129
L-Glutamic Acid Absent
L-Glutamine Absent
Glycine 0.01875
L-Histidine HCl•H2O 0.03148
L-Isoleucine 0.05447
L-Leucine 0.05905
L-Lysine•HCl Absent
L-Methionine Absent
L-Phenylalanine 0.03548
L-Proline 0.01725
L-Serine 0.02625
L-Threonine 0.05345
L-Tryptophan 0.00902
L-Tyrosine•2Na•2H20 0.05579
L-Valine 0.05285
Vitamins:
Biotin 0.0000035
Choline Chloride 0.00898
VitaminB12 0.00068
Folic Acid 0.00265
i-inositol 0.0126
Niacinamide 0.00202
D-Pantothenic Acid•Ca 0.00224
Pyridoxal Hydrochloride 0.002
Pyridoxine HCl 0.000031
Riboflavin 0.000219
Thiamine HCl 0.00217
Other:
Thymidine 0.000365
D-Glucose 3.151
HEPES 3.5745
Hypoxanthine 0.00239
Linoleic Acid 0.000042
Lipoic Acid 0.000105
Phenol Red Absent
Putrescine•HCl 0.000081
Pyruvic Acid,Sodium 0.055
Total:12.24g/Liter
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter: Dissolve 15.25g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off- white to white, homogeneous, free flowing powder.

Solubility: Colorless to pale yellow, clear, complete

pH: As reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).