Technical Data
D9901-20
Dynamin, phosphorylated (Ser774)
Description:
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:1000
Western Blot: 1:1000; Band at ~95kD. The labeling of the dynamin band in Western Blots of rat brain was blocked by the phosphopeptide used as the immunogen, but not by the corresponding dephosphopeptide.
Immunocytochemistry: 1:1000
Immunohistochemistry: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain synaptosomal lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanSheep
Concentration:
Not Determined
Immunogen:
Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser774 of human, mouse and rat dynamin.
Purity:
Purified by Protein A and immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes human dynamin. Labels dynamin phosphorylated in vitro by Cdk5, but not PKC. Does not crossreact with other purified substrates of Cdk5 (eg. amphiphysin and synapsin). Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tan, T.C., et al., Nat. Cell Biol. 8: 701-710 (2003). 2. Graham, M.E., et al., Proc. Natl. Acad. Sci. USA 99: 7124-7129 (2002). 3. Tsuboi, T., et al., J. Biol. Chem. 277: 15,957-15,961 (2002). 4. Cousin, M.A., et al., J. Neurochem. 76: 105-116 (2001).