Technical Data
E2F1 Transcription Factor (E2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3)
Recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

Positive Control: E2F-1 transfected Soas2 cells and recombinant fusion protein.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceHumanMouse
As reported
Fusion protein containing the region encoding the transactivation and retinoblastoma binding domain (amino acids 332-390) of E2F-1 expressed in E. coli.
Mouse ascites
As reported
Specific for human E2F-1. Species Crossreactivity: human E2F-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Shan, B., et al., (1994) Mol. Cell. Biol. 14:299-304. 2.Shan, B., et al., (1996) Proc. Natl. Acad. Sci. USA 93:679-684. 3.Shan, B., et al., (1996) Cell Growth and Diff. 7:689-697.