Technical Data
Molecular Biology Storage: -20CShipping: Blue Ice
5'-G A C N N N^N N G T C-3'
3'-C T G N N^N N N C A G-5'

Concentration: 10u/ul

Source: Enterobacter amnigenus RFL1105

Supplied with:
R1625-50: Restriction Enzyme Buffer, 10X for E0200-05
Supplied in a 10X concentrated stock. Dilute to 1X or 2X before use.
When used as a 1X liquid the form is 10mM Tris-HCl, 5mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA. Incubate at 37C

R1625: Restriction Enzyme Buffer A, 10X
Supplied in a 10X concentrated stock. Dilute to 1X or 2X before use.
When used as a 1X liquid the form is 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA

Storage Buffer:
Supplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion Assay:
No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours).

Ligation/Recutting Assay:
The ligation and recutting assay was replaced with LO test ater validating experiments showed LO test ability to trace nuclease and phosphate activities with sensitivity that is higher than L/R by a factor of 10.

Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of E0200-05 for 4 hours.

Star Activity:
An excess of enzyme (7.5u/ug DNA x 16 hours) may result in star activity.

Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C.

Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min.

Digestion of Agarose-embedded DNA: A minimum of 5 units of Eam11051 is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.

Number of Recognition Sites in DNA:
Lambda: 9
PhiX174, pTZ19R/U, pBR322, pUC18/19, pUC57: 1
M13mp18/19: 0

Enzyme Properties:
Methylation Effects on Digestion:
Dam, Dcm, EcoKl, EcoBl: never overlaps - no effect
CpG: May overlap - no effect
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.