Technical Data
E0200-05
Eam1105I
1000u
5000u
5000u
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Technical Data |
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E0200-05 |
Eam1105I |
1000u 5000u |
| Molecular Biology | Storage: -20°CShipping: Blue Ice |
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5'-G A C N N N^N N G T C-3' 3'-C T G N N^N N N C A G-5' Concentration: 10u/ul Source: Enterobacter amnigenus RFL1105 Supplied with: R1625-50: Restriction Enzyme Buffer for E0200-05, 10X 10mM Tris-HCl (pH 7.5), 5mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA. Incubate at 37°C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. |
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours). Ligation/Recutting Assay: After 16-fold overdigestion (1u/ug DNA x 16 hours) with Eam1105I, more than 80% of the pUC19 DNA fragments can be ligated in a reacton mixture containing 20–40u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5'-termini concentration of 1.8uM. More than 90% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Star Activity: An excess of enzyme (7.5u/ug DNA x 16 hours) may result in star activity. Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Thermal Inactivation: Enzyme is inactivated by incubation at 65°C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. |
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