Technical Data
E2000-25
ECSIT (Evolutionarily Conserved Signaling Intermediate in Toll Pathways)
Description:
Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Applications:
Can be used for detection of ECSIT by Western blot at 0.5 to 1ug/ml.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Peptide corresponding to 14 amino acids near the C-terminus of human TRIF (GenBank accession no. NP_057665)
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity:
Recognizes human ECSIT. Species Crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Takeda K, Kaisho T, and Akira S., (2003) Annu. Rev. Immunol. 21:335-76. 2. Vogel SN, Fitzgerald KA, and Fenton MJ., (2003) Mol. Interv. 3:466-77. 3. Janssens S and Beyaert R., (2003) Mol. Cell. 11:293-302. 4. Sato S, et al., (2003) J. Immunol. 171(8):4304-10. 5. Kopp E, et al., (1999) Genes Dev. 13:2059-71. 6. Xiao C, et al., (2003) Genes Dev. 17:2933-49.