Technical Data
Media
Edinburgh Minimal Media Phosphate Free (EMMP) (Powder)
E2205-20
Molecular Biology Grade
Storage RT/4°C    Shipping RT
Components shown as g/Liter
Base:
Ammonium Chloride5.000
Glucose20.000
Sodium Acetate•3H2O2.200
Salt Stock:
Calcium Chloride•2H2O0.0147
Magnesium Chloride•6H2O1.050
Potassium Chloride1.000
Sodium Sulfate0.040
Vitamin Stock:
Biotin 0.00001
Inositol0.010
Nicotinic Acid0.010
Pantothenic Acid•Ca0.001
Mineral Stock:
Boric Acid0.0005
Citric Acid0.001
Copper Sulfate•5H2O 0.00004
Ferrous Chloride 0.0002
Manganese Sulfate0.0004
Potassium Iodide0.0001
Molybdic Acid 0.00004
Zinc Sulfate•7H2O0.0004
Total: 29.328g/L
Note: The above formulation was derived from the following referenced formula:
Edinburgh Minimal Medium (EMM 2)
3g/LPotassium hydrogen phthalate (14.7mM)
2.2g/LSodium phosphate dibasic (15.5mM)
5g/LAmmonium chloride (93.5mM)
2% (w/v)Glucose (111mM)
20ml/LSalts (50X stock)
1ml/LVitamins (1000X stock)
0.1ml/LMinerals (10,000X stock)
50X Salts:
52.5g/LMagnesium chloride•6H2O (0.26M)
0.735g/LCalcium chloride•2H2O (4.99mM)
50g/LPotassium chloride (0.67M)
2g/LSodium sulfate (14.1mM)
1000X Vitamins:
1g/LPantothenic acid (4.20mM)
10g/LNicotinic acid (81.2mM)
10g/LInositol (55.5mM)
10mg/LBiotin (40.8uM)
10,000X Minerals:
5g/LBoric acid (80.9mM)
4g/LManganese sulfate (23.7mM)
4g/LZinc sulfate•7H2O (13.9mM)
2g/LFerrous Chloride (7.40mM)
0.4g/LMolybdic acid (2.47mM)
1g/LPotassium iodide (6.02mM)
0.4g/LCupric sulfate.5H2O (1.60mM)
10g/LCitric acid (47.6mM)
Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe. Phosphate Free formulation is used for 32P-phosphate labeling.

Sodium phosphate is removed and replaced with potassium hydrogen phthalate with 2g/L sodium acetate (14.6mM). The medium is adjusted to pH 5.5.


Directions per Liter: Dissolve 29.328 grams per liter of ddH2O. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi). Note: May also sterilize with 0.2um membrane filter. Aliquot into sterile containers. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to light tan, homogeneous, free flowing powder

Solubility: Colorless, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.