Technical Data

Media

Edinburgh Minimal Media Phosphate Free (EMMP) (Powder)

E2205-20

Molecular Biology Grade

Storage RT Shipping RT

Components shown as g/Liter

Base:
Ammonium Chloride	5.000
Glucose	20.000
Sodium Acetate•3H2O	2.200
Salt Stock:
Calcium Chloride•2H2O	0.0147
Magnesium Chloride•6H2O	1.050
Potassium Chloride	1.000
Sodium Sulfate	0.040
Vitamin Stock:
Biotin	0.00001
Inositol	0.010
Nicotinic Acid	0.010
Pantothenic Acid	0.001
Mineral Stock:
Molybdic Acid	0.00004
Boric Acid	0.0005
Citric Acid	0.001
Copper Sulfate •5H2O	0.00004
Ferrous Chloride 0.0002
Manganese Sulfate	0.0004
Potassium Iodide	0.0001
Zinc Sulfate•7H2O	0.0004

Total:	29.328g/Liter

Note: The above formulation was derived from the following referenced formula:
Edinburgh Minimal Medium (EMM 2)
3g/L	Potassium hydrogen phthalate (14.7mM)
2.2g/L	Sodium phosphate dibasic (15.5mM)
5g/L	Ammonium chloride (93.5mM)
2% (w/v)	Glucose (111mM)
20ml/L	Salts (50X stock)
1ml/L	Vitamins (1000X stock)
0.1ml/L	Minerals (10,000X stock)

50X Salts:
52.5g/L	Magnesium chloride•6H2O (0.26M)
0.735g/L	Calcium chloride•2H2O (4.99mM)
50g/L	Potassium chloride (0.67M)
2g/L	Sodium sulfate (14.1mM)

1000X Vitamins:
1g/L	Pantothenic acid (4.20mM)
10g/L	Nicotinic acid (81.2mM)
10g/L	Inositol (55.5mM)
10mg/L	Biotin (40.8uM)

10,000X Minerals:
5g/L	Boric acid (80.9mM)
4g/L	Manganese sulfate (23.7mM)
4g/L	Zinc sulfate•7H2O (13.9mM)
2g/L	Ferrous Chloride (7.40mM)
0.4g/L	Molybdic acid (2.47mM)
1g/L	Potassium iodide (6.02mM)
0.4g/L	Cupric sulfate.5H2O (1.60mM)
10g/L	Citric acid (47.6mM)

Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe. Phosphate Free formulation is used for 32P-phosphate labeling.

Sodium phosphate is removed and replaced with potassium hydrogen phthalate with 2g/L sodium acetate (14.6mM). The medium is adjusted to pH 5.5.

Directions per Liter: Dissolve 29.328 grams per liter of ddH2O. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi). Note: May also sterilize with 0.2um membrane filter. Aliquot into sterile containers. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to light tan, homogeneous, free flowing powder

Solubility: Colorless, clear, complete

pH: As reported

Stability: Stable up to 6 months

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.