Technical Data
E2208-01F
EDG1 (Endothelial Cell Differentiation Gene 1, EDG 1, EDG-1, Endothelial Differentiation Sphingolipid G Protein Coupled Receptor 1, CHEDG1, D1S3362, G Protein Coupled Sphingolipid Receptor, Sphingosine 1-phosphate Receptor Edg-1, Sphingosine 1-phosphate R
Description:
EDG1, a Lysophospholipid/Lysosphingolipid Receptor, binds sphingosine-1-phosphate and may control cell proliferation, survival, and suppression of apoptosis. Overexpression of EDG-1 leads to increased cell-cell aggregation and overexpression of cadherins. EDG-1 transcripts have been reported to be present in embryonic and adult brain, lung alveolar macrophages, vascular smooth muscle cells, endothelial cells, fibroblasts, melanocytes, and TAg-Jurkat T cells, but to be absent from breast cancer cell lines. ESTs have been isolated from brain, breast, heart, heart/melanocyte/uterus, kidney, liver, liver/spleen, lung, nerve, placenta, prostate, tonsil, uterus, and vessel libraries.

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:200
Western Blot: 1:100-1:1000
Immunohistochemistry (Paraffin): 1:50
Immunohistochemistry: 1:10-1:2000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma fused with spleen cells from a mouse immunized with human recombinant Endothelial Differentiation Gene 1 (EDG1) protein.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19G58Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml (after reconstitution)
Immunogen:
Human recombinant EDG-1 protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS. No preservatives added. Reconstitute with 500ul sterile PBS.
Specificity:
Recognizes human EDG1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.