Technical Data
E2230-13A
Elastase, Neutrophil
Description:
Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5kD.

Applications:
Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanSheep
Concentration:
~10mg/ml
Immunogen:
Neutrophil elastase (active-site blocked) prepared from human sputum.
Purity:
Purified by caprylic acid and ammonium sulfate precipitations, and solid -phase adsorption to remove unwanted crossreactivity.
Form
Supplied in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
Specificity:
Recognizes human Neutrophil Elastase. Monospecific when analyzed by immunoelectrophoresis. No reaction was seen with normal human plasma and only one band was observed in plasma to which active-site blocked elastase had been added. Identity was verified using purified elastase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.