Technical Data
E2236-40
Elastin, alpha (alpha Elastin, Elastin Isoform a, ELN, FLJ38671, FLJ43523, Supravalvular Aortic Stenosis, SVAS, WBS, WS)
Description:
Elastin is a major structural protein expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus. It is also expressed in the large arteries, lung and skin.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:8000. The 50% OD was observed using 1ug of native antigen/well. Under the same conditions, the end-point was observed at 1:200,000.
Immunohistochemistry (Paraffin): Fixation with 10% formalin is recommended. Brief digestion with hyaluronidase or trypsin aids antigen retrieval.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanRabbit
Concentration:
~5mg/ml
Immunogen:
Human alpha Elastin
Purity:
Purified by Protein G affinity chromatography
Form
Supplied as a liquid in PBS, 0.09% sodium azide
Specificity:
Recognizes human alpha Elastin. Species Crossreactivity: bovine and tree shrew
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yu, J., et al., The elastic network of articular cartilage: an immunohistochemical study of elastin fibres and microfibrils. J. Anat. 216: 533-541 (2010). 2. Grant, T.M., et al., Elastic fibres are broadly distributed in tendon and highly localized around tenocytes. J. Anat. 222: 573-579 (2013). 3. Albon, J., et al., Connective tissue structure of the tree shrew optic nerve and associated ageing changes. Invest. Ophthalmol. Vis. Sci. 48: 2134-2144 (2007).