Technical Data
Elastin, alpha (alpha Elastin, Elastin Isoform a, ELN, FLJ38671, FLJ43523, Supravalvular Aortic Stenosis, SVAS, WBS, WS)
Elastin is a major structural protein expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus. It is also expressed in the large arteries, lung and skin.

Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:8000. The 50% OD was observed using 1ug of native antigen/well. Under the same conditions, the end-point was observed at 1:200,000.
Immunohistochemistry (Paraffin): Fixation with 10% formalin is recommended. Brief digestion with hyaluronidase or trypsin aids antigen retrieval.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
1ml-20CBlue IceHumanRabbit
Human alpha Elastin
Purified by Protein G affinity chromatography
Supplied as a liquid in PBS, 0.09% sodium azide
Recognizes human alpha Elastin. Species Crossreactivity: bovine and tree shrew
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yu, J., et al., The elastic network of articular cartilage: an immunohistochemical study of elastin fibres and microfibrils. J. Anat. 216: 533-541 (2010). 2. Grant, T.M., et al., Elastic fibres are broadly distributed in tendon and highly localized around tenocytes. J. Anat. 222: 573-579 (2013). 3. Albon, J., et al., Connective tissue structure of the tree shrew optic nerve and associated ageing changes. Invest. Ophthalmol. Vis. Sci. 48: 2134-2144 (2007).