Technical Data
E2275-06
ENA78, Recombinant, Human (Epithelial Neutrophil Activating Peptide 78)
5ug
20ug
Growth Factors, Cytokines Storage: -20°CShipping: Blue Ice
Recombinant Human ENA-78 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8020 Dalton. Recombinant Human ENA-78 is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-Arg. .

Dimers and Aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
Recombinant Human ENA-78 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human peripheral blood neutrophils using a concentration of 5-10 ng/ml.

Endotoxin:
0.1ng/ug (IEU/ug) of Recombinant Human Epithelial neutrophil-activating protein 78

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a calibrated solution of ENA-78 as a Reference Standard.

Solubility:
It is recommended to reconstitute the lyophilized ENA-78 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized ENA-78 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution ENA-78 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Molecular Weight:
8.02kD
 Source: E. coli
Purity: 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder without additives.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.