Technical Data
E2286
HERV (HERV-W_7q21.2 Provirus Ancestral Env Polyprotein, Envelope Polyprotein gPr73, HERV-7q Envelope Protein, HERV-W Envelope Protein, Syncytin, Syncytin-1, Enverin, Env-W, ERVWE1)
Description:
HERVs are a class of multiple sclerosis associated retroviruses. Reacts with a replication competent Human Endogenous Retrovirus (HERVW) isolated from placenta. The synthetic peptide sequence used as immunogen is taken from the virus envelope polyprotein sequence. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. The HERVW endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:250-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa42-50 (QRPGNIDAPC) of human HERV protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes a replication-competent human Endogenous Retrovirus (HERV) isolated from placenta. Recognizes aa42-50 (QRPGNIDAPC) of the HERV protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Blond, et al., (1999) J. Virol. 73:11751185.