Technical Data
Endomorphin 1
Endomorphins are recently discovered endogenous opioid peptides composed of four amino acids: Endomorphin-1 (EM-1) is Tyr-Pro-Trp-Phe-NH2, and Endomorphin-2 (EM-2) is Tyr-Pro-Phe-Phe-NH2. These peptides have a very high affinity and specificity for mu-opioid receptors as it has been reported in many pharmacological studies. EM-1 is distributed in many brain regions, whereas EM-2 is prevalent in brainstem and spinal cord. EM-1 and EM-2 exert their antinociceptive activity by interacting with mu-opioid receptors. EM-1 anf EM-2 bind with mu-opioid receptor with high affinity and selectivity in comparison with binding to opioid delta and kappa receptors. EM-1 and EM-2 tetrapeptides activate G-proteins and inhibit adenylyl cyclase activity when bind to mu-opioid receptors.

Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Immunofluorescence): 15mg/ml
Immunohistochemistry (Avidin-Biotin): 5mg/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
50ul-20CBlue IceRabbit
Synthetic peptide corresponding to Endomorphin 1, Tyr-Pro-Trp-Phe-NH2 (BSA)
Purified by affinity chromatography.
Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O, 0.09% sodium azide..
Recognizes Endomorphin 1. Displays 5% crossreactivity with Endomorphin 2, <0.03% with Met5-Enkephalin and <0.01% with Leu5-Enkephalin and beta-Endorphin. Species Crossreactivity: rat, mouse, human, porcine and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.