Technical Data
E2291-75C
Endoplasmic Reticulum Protein 57, CT (ERp57, GRP58, PDIA3, Protein Disulfide Isomerase Family A, Member 3 [Homo sapiens], HGNC:4606, ERp60, ERp61, GRP57, HsT17083, P58, PI-PLC, 58kD Microsomal Protein, Endoplasmic Reticulum P58, Glucose Regulated Protein,
Description:
ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin
(lectin chaperones) via their proline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

Applications:
Suitable for use in Western Blot and Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot:1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110F699Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human recombinant ERp57 (GRP58).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.4, in 0.09% sodium azide and 50% glycerol.
Specificity:
Recognizes human ERp57. Species Crossreactivity: bovine, canine, guinea pig, hamster, monkey, porcine, rabbit, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87:1377-1408. 2. Williams D.B. (2005) J Cell Sci. 119: 615-623 3. Maattanen P., et al. (2006) Biochem Cell Biol. 84:881-889. 4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582. 5. Oliver J.D., et al. (1997) Science 275: 86-88. 6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226. 7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230. 8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305. 9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.