Technical Data
E3015-04
Endothelial Monocyte Activating Polypeptide II (EMAP II)
Description:
EMAP II is an antiangiogenic and proinflammatory cytokine (expressed by microglia/macrophages). EMAP II appears to be a profound modulator of inflammatory reactions of the innate immune system and might participate in a variety of pathogenic immune-processes in the mammalian brain. EMAP II is a potential mediator of inflammatory responses in autoimmunity and a considered target to stage reactions of monocytic lineage cells in inflammatory reactions. In normal tissues EMAP II is expressed in endocrine organs, in cells of neuroendocrine origin, but also in tissues with high cell turnover. Under pathophysiological conditions EMAP II has several functions. It triggers the recruitment of macrophages by elevating cytosolic-free Ca2+ concentrations, it stimulates leukocyte chemotaxis, it induces the expression of TNF-alpha and tissue factor (TF) by monocytes and myeloperoxidase (MPO) by polymorphonuclear cells (PNMs). Furthermore it stimulates the release of von Willebrand-factor, P- and E-selectin by endothelial cells and EMAP II induces endothelial programmed cell death in vitro.

Applications:
Suitable for use in Western Blot, Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistology (Paraffin): 1:10
Flow Cytometry: 1:10
Western Blot: 1:10
ELISA: Detection
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b6A542Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Human Endothelial Monocyte Activating Polypeptide II (EMAP II).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.
Specificity:
Recognizes human endothelial-monocyte-activating polypeptide II (EMAP II) at ~20kD. Species Crossreactivity: Rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mueller, C et al, (2003) Neurosci Lett 339:215. 2. Mueller, C et al, (2003) Neuroimmunol 135:1. 3. Mueller, C et al, J Neurotrauma 20:1007. 4. Schluesener, H et al, (1997) Glia 20:365.