Technical Data
E3098-29B
Endothelin 1
Description:
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms, ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart and liver.

Applications:
Suitable for use in Immunohistochemistry and the development of IRMA assays for endothelin ET-1 which as a circulating plasma peptide has been associated with a variety of disease studies such as diabetes, hypertension, cardiogenic shock, acute myocardial infarction and uraemia. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q2188Affinity Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Tissue culture supernatant.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10mM sodium azide.
Specificity:
Recognizes human endothelin ET-1 (10% crossreactivity with ET-3) and when used in conjunction with anti-endothelin, C-terminal, has a sensitivity of 0.06pmol/l for ET-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.