Technical Data
E3100
Endothelin A Receptor, aa410-422 (ET-A, ET1-Specific Type Endothelin Receptor, Ednra, ETA, ETRA)
Description:
The receptors for the endothelins are found on various tissues including lung and intestine. ETA receptor has been reported in a variety of tissues including placenta, uterus, testis, heart, adrenal gland, lung, kidney, ovary, skin, liver, bladder, large blood vessels, cerebellum, neurons, and meningioma tumor cells. A splice variant has been identified in melanoma (Zhang et al., 1998). ESTs have been isolated from libraries from the above-mentioned tissues.

Applications:
Suitable for Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Suitable for paraffin embedded tissue.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceRatSheep
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa410-422, QEQNHNTERSSHK of rat Endothelin A Receptor (C-Terminal-KLH). MW: 39kD.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5. No preservative added.
Specificity:
Recognizes rat Endothelin A Receptor. Tested using endothelin A receptor transfected COS cells for the expression of the 39kD segment of the cytoplasmic region of the receptor. Species Crossreactivity: human, mouse and sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chun, M., et al. (1995) J. Biol. Chem. 220:10,855-10,860. 2. Fernandes, L.B., et al., (1998) Journal of Cardiovascular Pharmacology 31:S222-S224 Supplement 1.