		Technical Data
E3100	Endothelin A Receptor, aa410-422 (ET-A, ET1-Specific Type Endothelin Receptor, Ednra, ETA, ETRA)

Description:
The receptors for the endothelins are found on various tissues including lung and intestine. ETA receptor has been reported in a variety of tissues including placenta, uterus, testis, heart, adrenal gland, lung, kidney, ovary, skin, liver, bladder, large blood vessels, cerebellum, neurons, and meningioma tumor cells. A splice variant has been identified in melanoma (Zhang et al., 1998). ESTs have been isolated from libraries from the above-mentioned tissues.

Applications:
Suitable for Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Suitable for paraffin embedded tissue.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
250ug		-20°C	Blue Ice	Rat	Sheep

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to aa410-422, QEQNHNTERSSHK of rat Endothelin A Receptor (C-Terminal-KLH). MW: 39kD.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.5. No preservative added.

Specificity:

Recognizes rat Endothelin A Receptor. Tested using endothelin A receptor transfected COS cells for the expression of the 39kD segment of the cytoplasmic region of the receptor. Species Crossreactivity: human, mouse and sheep.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Chun, M., et al. (1995) J. Biol. Chem. 220:10,855-10,860. 2. Fernandes, L.B., et al., (1998) Journal of Cardiovascular Pharmacology 31:S222-S224 Supplement 1.