Technical Data
E3362-01J
EphA4 (Ephrin Receptor Eph A4, Ephrin Receptor EphA4, Ephrin Type A Receptor 4, EPH Receptor A4, Eph A4, EPH-like Kinase 8, EK8, HEK8, Receptor Protein Tyrosine Kinase HEK8, SEK, TYRO1 Protein Tyrosine Kinase, Tyrosine Protein Kinase Receptor SEK, Tyrosin
Description:
EphA4 is ubiquitously expressed in the body, and is thought to play a role in a number of different developmental pathways, including the corticospinal tract formation, hindbrain pattern formation and regulating the structure of synaptic connections. EphA4 may also act as a locomotor central pattern generator.

Applications:
Suitable for use in Immunohistochemistry (paraffin). Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 5-10ug/ml
Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher.

Positive Control: Glomerulus

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to internal domain of human EphA4.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.7, 0.09% sodium azide.
Specificity:
Recognizes human EphA4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kullander, K. et al. (2001) Kinase-dependent and kinase-independent functions of EphA4 receptors in major axon tract formation in vivo. Neuron 29: 73-84. 2. Murai, K. K. et al. (2003) Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling. Nat. Neurosci. 6: 153-160. 3. Theil, T. et al. (1998) Segmental expression of the EphA4 (Sek-1) receptor tyrosine kinase in the hindbrain is under direct transcriptional control of Krox-20. Development. 125: 443-452.