		Technical Data
E3372	Ephrin B3 (EFNB3, Ephrin B3 (EFL6, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8, Ephrin-B3, EPLG8, LERK8)

Description:
Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family. Both ephrins and Eph receptors are broadly expressed throughout the ectoderm, mesoderm and endoderm of vertebrate embryos (1). The interaction of ephrins with the appropriate Eph receptor(s) has been implicated in a wide spectrum of functions in vertebrate morphogenesis, including the accurate migration of embryonic cells (2), formation of boundaries between structures (i.e. rhombomeres and somites) and the control of cytoskeletal changes which dictate cellular shape and adhesion during development. Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors; Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors. Ephrin-B3 is a Class B protein. It has been shown to bind the EphB1, EphB2, EphB3 and EphA4 receptors (4).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-5ug/ml.
Immunoprecipitation: 5-10ug/ IP reaction
Immunohistochemistry (FFPE): 5-10ug/m. Confirmed with human tissue sections. Enzyme digestion with pepsin is required for optimal staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide derived from an internal sequence of the mouse ephrin-B3 protein.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Specificity:

Recognizes mouse ephrin-B3 protein. Species Crossreactivity: human. Reactivity is con rmed with a chimeric protein consisting of the extracellular domain of human ephrin-B3 and the Fc region of human IgG1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Holder N, Klein R. Development 126(10):2033-2044, (1999). 2. Flanagan JG, Vandehaeghen P. Annu Rev Neurosci 21:309-345, (1998). 3. Unified nomenclature for Eph family receptors and their ligands, the ephrins. Eph Nomenclature Committee. Cell 90(3):403-404, (1997). 4. Pasquale EB. Curr Opin Cell Biol 9(5):608-615, (1997).