Technical Data
E3374-03B
Epidermal Growth Factor, Recombinant, Human (E. coli) (EGF)
100ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Recombinant human epidermal growth factor(EGF) is a 6.3kD globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing.

Source:
Recombinant human Epidermal Growth Factor, aa971-1023, expressed in E. coli.

Molecular Weight: 6.2kD

Biological Activity:
ED50 is < 0.1ng/ml. ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells.

Endotoxin: 1EU/ug (LAL)

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
6.2kD
Source: E. coli
Purity: 98% (SDS-PAGE and HPLC)
Concentration: As reported
Form: Supplied as a lyophilized powder. Reconstitute with ddH2O or PBS.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. GAPex-5 mediates ubiquitination, trafficking, and degradation of epidermal growth factor receptor J. Biol. Chem. 282 (29), 21278-21284 (2007). 2. Variant genotypes and haplotypes of the epidermal growth factor gene promoter are associated with a decreased risk of gastric cancer in a high-risk Chinese population Cancer Sci. 98 (6), 864- 868 (2007).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.