Technical Data
Epidermal Growth Factor, Recombinant, Human (E. coli) (EGF)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant human epidermal growth factor(EGF) is a 6.3kD globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing.

Recombinant human Epidermal Growth Factor, aa971-1023, expressed in E. coli.

Molecular Weight: 6.2kD

Biological Activity:
ED50 is < 0.1ng/ml. ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells.

Endotoxin: 1EU/ug (LAL)

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% (SDS-PAGE and HPLC)
Concentration: As reported
Form: Supplied as a lyophilized powder. Reconstitute with ddH2O or PBS.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. GAPex-5 mediates ubiquitination, trafficking, and degradation of epidermal growth factor receptor J. Biol. Chem. 282 (29), 21278-21284 (2007). 2. Variant genotypes and haplotypes of the epidermal growth factor gene promoter are associated with a decreased risk of gastric cancer in a high-risk Chinese population Cancer Sci. 98 (6), 864- 868 (2007).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.