Technical Data
E3374-07
Epidermal Growth Factor, Murine (EGF)
100ug
Molecular Biology Storage: -20CShipping: RT
Biological Activity: The optimal EGF concentration in cell culture is 0.125ng/ml depending on cell type.

Biological Activity: 5ng/ml of this lot supports a 2.5- fold increase in cell number in a 5-day assay using human foreskin broblasts. Previously, 5ng/ml EGF supported a 2.2-fold increase in cell number in a 5-day assay using normal rat kidney broblasts (NRK-49F).

Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
16kDa
Source: Mouse
Purity: Single band on SDS-PAGE gel at 16kDa (1ug run under non-reducing conditions and Coomassie blue stained).
Form: 100ug/vial of lyophilized powder. Lyophilized from 157ul of 20mM ammonium acetate. Quantitated by spectrophotometry using the extinction coef cient for EGF [E (1%, 280nm)]= 30.9

Important Note: This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.