Technical Data
E3374-07
Epidermal Growth Factor, Murine (EGF)
100ug
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Technical Data |
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E3374-07 |
Epidermal Growth Factor, Murine (EGF) |
100ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Biological Activity: The optimal EGF concentration in cell culture is 0.1–25ng/ml depending on cell type. Biological Activity: 5ng/ml of this lot supports a 2.5- fold increase in cell number in a 5-day assay using human foreskin broblasts. Previously, 5ng/ml EGF supported a 2.2-fold increase in cell number in a 5-day assay using normal rat kidney broblasts (NRK-49F). Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 16kDa |
Source: Mouse Purity: Single band on SDS-PAGE gel at 16kDa (1ug run under non-reducing conditions and Coomassie blue stained). Form: 100ug/vial of lyophilized powder. Lyophilized from 157ul of 20mM ammonium acetate. Quantitated by spectrophotometry using the extinction coef cient for EGF [E (1%, 280nm)]= 30.9 Important Note: This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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