Technical Data
E3375-03
Epidermal Growth Factor Receptor (erbB, EGFR), Extracellular Domain
Description:
EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to epidermal growth factor (EGF), transforming growth factor-a
(TGFa), heparin binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. It is predominantly present in squamous cell carcinomas.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Affinity Purification, Blocking of EGF/TGFa-induced Activation of EGFR, Inhibition of Cell Proliferation (A431 cells), Arrests tumor growth in vivo. Not suitable for Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation: Native only.
Affinity Purification: Recommended to use E3375-03X
Blocking of EGF/TGFa-induced Activation of EGFR: 0.5ug/ml, cells induced by 30ng/ml EGF. Recommended to use E3375-03X.
Arrests tumor growth in vivo: Recommended to use E3375-03X.
Optimal dilutions to be determined by the researcher.

Positive Control:
A431 cells

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a0.N.265Affinity Purified
SizeStorageShippingSourceHost
500ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purified EGFR from A431 cells. Cellular Localization: Cell membrane.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15mM sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See E3375-03X.
Specificity:
Recognizes the 170kD (intact) and 145kD (VIII variant) forms of EGF receptor. The epitope maps to the extracellular domain of EGFR. Blocks EGF/TGFa-induced activation of EGFR (1,2). No effect on tyrosine kinase activity of the receptor. Inhibits the binding of antibody E3375-04 (-04X). Does not crossreact with erbB-2, erbB-3, or erbB-4. Species crossreactivity: Human and canine. Does not cross react with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kawamoto, T., et al., (1983) PNAS USA 80:1337-41. 2. Sato, J.D., et al., (1983) Molecular Biology and Medicine 1(5):511-29. 3. den Hartigh, J.C., et al., (1993) Biochimica et Biophysica Acta 1148(2):249-56. 4. Wikstrand, C.J., et al., (1995) Cancer Research 55(14):3140-8.