Technical Data
E3375-16
Epidermal Growth Factor Receptor (erbB, EGFR) (nonPhospho-Y1173)
Description:
Applications:
Suitable for use in Western Blot, Immunoprecipitation.

Recommended Dilution:
Western Blot: 0.5-2ug/ml detects the EGF receptor (unphosphorylated at Y1173) in RIPA lysates from unstimulated HFF cells. Lysates from EGF stimulated and unstimulated HFF cells, 10ug per lane, were resolved by electrophoresis and transferred to nitro-cellulose. Lanes 1 and 2 were probed with anti-EGF receptor and visualized using a rabbit anti-sheep secondary antibody conjugated to HRP. Lanes 3 and 4 were probed with anti-EGF-R (non-phospho-Y1173) (1ug/ml) and visualized using a goat anti-mouse secondary antibody conjugated to HRP. A chemiluminescence detection system was used. (EGF-R 175 kD).

Immunoprecipitation: 1-10ug of this antibody is reported to immunoprecipitate the EGF receptor (unphosphorylated at Y1173) from 10e6 vanadate treated A431 cells.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k0.T.197Affinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
1mg/ml
Immunogen:
KLH-conjugated, synthetic peptide containing the unphosphorylated Tyr1173 motif (… NAE[Y]LR… ) of the human EGF receptor.
Purity:
Purified by subsequent thiophilic adsorption and size exclusion chromatography
Form
Supplied as a liquid in 0.07M Tris-glycine, pH 7.4, 0.105M sodium chloride, 0.05% sodium azide, 30% glycerol. Also available without azide and glycerol. See E3375-16X.
Specificity:
Recognizes the motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173). Does not recognize the phosphorylated (pTyr1173) form of the EGF receptor. Reacts with human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sorkin, A., et al., (1992) J. Biol. Chem. 267:8672-8678. 2. Bertics, P., et al., (1988) J. Biol. Chem. 263:3610-3617.