Description:
EBV early antigen is the first detectable marker of EBV infection in human cells
Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 10C121 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 200ug | | -20°C | Blue Ice | | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Affinity purified early antigen polypeptides precipitated from African Burkitt's Lymphoma. |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. |
| Specificity: |
| Recognizes the the p50:p55 polypeptide complex associated with the early antigen of Epstein-Barr virus (EBV). Produces an intense diffuse and speckled staining pattern detectable in the nucleus of paraformaldehyde/acetone fixed cells expressing the EBV early nuclear antigen. Acetone and methanol fixation can be used, but the results are a less bright, coarsely granular nuclear staining pattern. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Epstein, A.L. et al. (1984) Immunobiochemical characterization with monoclonal antibodies of Epstein-Barr virus-associated early antigens in chemically induced cells. J. Virol. 50: 372-379.
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