Technical Data
E3451-35
ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian, HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3)
Description:
c-erbB-3/HER-3 is a member of the EGF receptor tyrosine kinase family. It is a kinase defective receptor which signals by forming heterodimers with other erb family members. c-erbB-3 is over-expressed in a variety of tumors including breast, stomach, pancreas and colon, although the significance is unclear because over-expression of c-erbB-3 has no effect on tumorigenic growth of cells. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. This antibody can be used to assess the contribution of the other erb family members co-expressed with c-erbB-3 to ligand-dependent signaling because it inhibits the binding of heregulin (NDF) to c-erbB-3/HER-3.

Applications:
Suitable for use in Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 10ug/ml shows positive immunostaining for erbB-3 in MCF-7 cells fixed with 95% ethanol/5% acetic acid.
Western Blot: 0.1-2ug/ml detects erbB-3 (~185kD) in RIPA lysates of human MCF-7 cells; previous lots have detected erbB-3 in MDA RIPA lysates. MCF-7 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E3451-35. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunoprecipitation: 4ug antibody immunoprecipitates erbB-3 from human MDA and MCF-7 RIPA lysates.
Immunohistochemistry: 1:50-1:100 dilution of a previous lot detected erbB-3 membrane staining in human normal and carcinoma breast tissue.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k0.T.39Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Recombinant rat erbB-3/HER-3 oncoprotein expressed in insect cells and purified by metal chelating chromatography. Epitope maps between aa1295-1323 of human erbB-3 (NM_001982).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes human erbB-3 (~185kD). Does not crossreact with erbB-1 (EGFR) and erbB-2/HER-2. Species Sequence Homology: mouse, rat and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Keely, S.J. & Barrett, K.E., J. Mol. Biol. 274: 33,449-33,454 (1999). 2. Ezeh, P.I. & Farbman, A.I., J. Neurobiol. 37: 199-210 (1998). 3. Kraus, M.H., Proc. Natl. Acad. Sci. USA 86: 9193-9197 (1989). 4. Paolo, F., Mol. Cell. Bio. 14: 492500 (1994).