Technical Data
E3500-04K
Escherichia coli (E. coli)
Description:
Escherichia coli (E. coli) is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, or by preventing the establishment of pathogenic bacteria within the intestine. E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination. The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology. E. coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885, and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1ml-20°CBlue IceRabbit
Concentration:
~4mg/ml
Immunogen:
A mixture of all antigenic serotypes.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes E. coli and reacts with all "O" and "K" antigenic serotypes of E. coli. has been used versus serotypes O157:H7, O20, O125, 055, 0111 and K12 and will remove E. coli proteins from recombinant preparations. This antibody is not absorbed and cross­reacts with related Enterobacteriaceae.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Skladal, P. et al. (2012) Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 in Bioaerosols Electroanalysis 24: 539-46. 2. Skladal, P. et al. (2013) Electrochemical immunosensors for detection of microorganisms Int. J. Electrochem. Sci., 8 1635 - 49 3. Su, W.H. et al. (2013) Development of a chip-based multiplexed immunoassay using liposomal nanovesicles and its application in the detection of pathogens causing female lower genital tract infections. Taiwan J Obstet Gynecol. 52: 25-32. 4. Ho, T.Y. et al. (2013) Development of a novel bead-based 96-well filtration plate competitive immunoassay for the detection of Gentamycin. Biosens Bioelectron. 49C: 126-132. 5. Ong, L.C. et al. (2014) Bacterial imaging with photostable upconversion fluorescent nanoparticles.