Exodus 2, Recombinant, Human (SLC, Secondary Lymphoid-tissue Chemokine, 6Ckine, TCA4)
|Molecular Biology||Storage: -20°CShipping: Blue Ice|
SLC/CCL21, normally expressed in high endothelial venules and in T cell zones of spleen and lymph nodes, strongly attracts T cells and dendritic cells (DC). Secondary lymphoid tissue chemokine (CCL21/SLC) regulates the homing of naive T cells and dendritic cells that express CC-chemokine receptor 7 (CCR7) from distant sites to lymphoid tissue such as lymph nodes.
Recombinant Human Exodus-2 is also known as SLC (Secondary Lymphoid-Tissue Chemokine), TCA-4 (T-cell activation-4) , and 6Ckine. Recombinant CCL21 produced in E.Coli as a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12219 Dalton. Recombinant CCL-21 is purified by proprietary chromatographic techniques.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be, Ser-Asp-Gly-Gly-Ala.
Dimers and Aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Recombinant Human Exodus-2 is fully biologically active when compared to standard. The activity is determined by the chemoattract of human PBE (peripheral blood eosinophils) at a concentration between 0.1-10ng/ml.
0.1ng/ug (IEU/ug) of Exodus-2.
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of Exodus-2 as a Reference Standard.
It is recommended to reconstitute the lyophilized Exodus-2 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Exodus-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Exodus-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source: E. coli
Purity: 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form: Supplied as a lyophilized powder without additives.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.