Technical Data
E9100-01
Ezrin (Thr567), (Radixin (Thr564), Moesin (Thr558), phosphorylated
Description:
The ezrin, radixin and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton, and are involved in cell adhesion, membrane ruffling and microvilli formation (1). ERM proteins also undergo intra-or intermolecular interaction between the N-and C-terminal domains, which makes them exist in inactive, cytosolic monomers or dimers (2). Phosphorylation at a C-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin), which disrupts the N-and C-terminal association, may play a key role in modulating the conformation and function of ERM proteins (3,4). It has also been demonstrated that phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin.
Purity:
Purified by protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of human ezrin, radixin and moesin only when phosphorylated at Thr567, Thr564 and Thr558 respectively. Does not crossreact with other related phospho proteins such as merlin or band 4.1. Species Crossreactivity: mouse, rat, and monkey. Species sequence homology: xenopus
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274:34507-34510. 2. Mangeat, P. et al. (1999) Trends Cell Biol. 9:187-192. 3. Matsui, T. et al. (1998) J. Cell Biol. 140:647-657. 4. Gautreau, A. et al. (2000) J. Cell Biol. 150:193-203. 5. Tran Quang, C. et al. (2000) EMBO J. 19:4565-4576. 6. Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:7300-7305. 7. Wu, K. L. et al. (2004) . J. Biol. Chem. 279 (25):26280-26286. 8. Gatto, C. L. et al. (2003) J. Cell Biol. 162 (3):489-498. 9. John, G. R. et al. (2004) J. Neurosci. 24 (11):2837-2845. 10. Fievet, B. T. et al. (2004) J. Cell Biol. 164 (5):653-659.