Technical Data
E9100-21
Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)
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Technical Data |
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E9100-21 |
Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein) |
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Description: Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein enriched in microvilli and other cell surface structures. Its NH2- terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells. Comments: Excellent for staining of formalin/paraffin tissues. Epitope: aa362-585 Applications: Immunoprecipitation: (Native and denatured) (Use Protein G) (Ab 2ug/mg protein lysate) Western Blot: (0.5-1ug/ml for 2hrs at RT) (1,2) Immunohistology: (Formalin/paraffin) (1) (Use Ab 1-2ug/ml for 30 min at RT) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive Control: Raji cells. Lung. Cellular Localization: Cell membrane. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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