Technical Data
E9100-21
Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)
Description:
Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein enriched in microvilli and other cell surface structures. Its NH2- terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells.

Comments: Excellent for staining of formalin/paraffin tissues.

Epitope: aa362-585

Applications:
Immunoprecipitation: (Native and denatured) (Use Protein G) (Ab 2ug/mg protein lysate)
Western Blot: (0.5-1ug/ml for 2hrs at RT) (1,2)
Immunohistology: (Formalin/paraffin) (1) (Use Ab 1-2ug/ml for 30 min at RT) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Positive Control: Raji cells. Lung.

Cellular Localization: Cell membrane.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15G110Affinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
A recombinant fragment of human ezrin corresponding to aa362-585.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
Specificity:
Recognizes Ezrin. Species Crossreactivity: Human, Monkey, Bovine, Kangaroo, Rat, Mouse, and Hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Helander TS, et. al. (1996) Nature 382:265-8. 2. Bohling T, et. al. (1996) Am J Path 148:367-73. 3. Turunen O, et al. (1994) J Cell Bio 126:1445-53.