Technical Data
Ezrin, Radixin, Moesin discontinue see E9100-26
Ezrin, Radixin and Moesin are proteins that function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling and microvilli formation.

Suitable for use in Imunocytochemistry/Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry/Immunofluorescence: 1:100.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
KLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin.
Purified by Protein A affinity chromatography and peptide affinity chromatography.
Supplied as a liquid in 10mM HEPES, pH 7.5, 150 mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Recognizes human Ezrin/Radixin/Moesin. Does not cross-react with other related proteins such as merlin or band 4.1. Species Crossreactivity: monkey, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.