Technical Data
E9100-25
Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)
Description:
Applications:
Suitable for use in Western Blotting, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western blotting: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry: 1:100. Immunofluorescence
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150 mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity:
Recognizes Ezrin/Radixin/Moesin. These proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling and microvilli formation. This antibody does not cross-react with other related proteins such as merlin or band 4.1. Species Crossreactivity: Human, monkey, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.