Technical Data
E9100-25
Ezrin, Radixin, Moesin discontinue see E9100-26
Description:
Ezrin, Radixin and Moesin are proteins that function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling and microvilli formation.

Applications:
Suitable for use in Imunocytochemistry/Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry/Immunofluorescence: 1:100.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
KLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin.
Purity:
Purified by Protein A affinity chromatography and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150 mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity:
Recognizes human Ezrin/Radixin/Moesin. Does not cross-react with other related proteins such as merlin or band 4.1. Species Crossreactivity: monkey, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.