Technical Data
Ezrin (Cytovillin, Villin-2, p81, EZR, VIL2)
Ezrin is a member of the ERM family (ezrin, radixin, moesin) of proteins that link the cell membrane to the actin cytoskeleton and that are involved in cytoskeletal organization, cell motility and morphogenesis. The protein (586aa) consists of three domains: a membrane-binding aminoterminal domain, an extended alpha-helical domain, and a carboxyterminal domain that has an actin-binding site. Aberrantly high ezrin protein expression has been detected in the stromal cells of hemangioblastoma. Moreover, a high ezrin expression level is associated with immortalization and the increased proliferation rate of cells in vitro. It appears to play a role in the progression of tumors, such as the metastasis of osteosarcoma. Human Ezrin gene is localized to the end of the long arm of chromosome 6 (6q22-q27).

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100-1:750
Immunohistochemistry (formalin fixed paraffin embedded): 1:50-1:100
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Positive Control:
Human lung cell line

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceHumanMouse
Not determined.
Recombinant corresponding to aa362-585 of human ezrin. Cellular Localization: Cell membrane.
Supplied as a liquid, 0.05% sodium azide.
Recognizes human Ezrin. Species Crossreactivity: bovine, hamster, monkey, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bohling et al. Am J Pathol 148: 367, 1996. 2. Helander et al. Nature 382: 265, 1996. 3. Turunen et al. J cell Biol 126: 1445, 1994. 4. Markku et al. Hum Genet 103: 662, 1998.