Technical Data
F0015-02
Factor VII (F.VII, Stable Factor, Proconvertin)
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Technical Data |
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F0015-02 |
Factor VII (F.VII, Stable Factor, Proconvertin) |
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Description: Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis. The predominant splicing variant of Factor VII in normal liver corresponds to the 444aa precursor. After cleavage of a signal peptide (residues 1-38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Direct ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1ug/ml detected recombinant human Factor VII. Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human Factor VII. Immunohistochemistry: 8-25ug/ml using immersion fixed PHA-stimulated human peripheral blood mononuclear cells (PBMCs). Optimal dilutions to be determined by the researcher. Hybridoma: NS0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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