Technical Data
F0015-02
Factor VII (F.VII, Stable Factor, Proconvertin)
Description:
Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis. The predominant splicing variant of Factor VII in normal liver corresponds to the 444aa precursor. After cleavage of a signal peptide (residues 1-38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Direct ELISA. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1ug/ml detected recombinant human Factor VII.
Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human Factor VII.
Immunocytochemistry: 8-25ug/ml using immersion fixed PHA-stimulated human peripheral blood mononuclear cells (PBMCs).
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG17H85Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa39-444 from human Factor VII expressed in NSO cells (NP_062562). Species Sequence Homology: canine - 76%, mouse - 71%, rat - 70%, chicken - 57%
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes human Factor VII. Does not crossreact with recombinant human Kallikrein-1, -2, -3, -4, -5, -7, -8, -9, -10, -11, -12, -13, -14, -15, -B1, recombinant human HGFA, recombinant human Factor X, recombinant human Factor XI, recombinant human Thrombin, recombinant human uPA-1 or recombinant human PROC.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.