Technical Data
F0019-55
FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)
Description:
FAK is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Studies have shown that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine- phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.

Applications:
Suitable for use in Immunoprecipitation and Immunofluorescence. Not recommended for Western Blot.
Other applications have not been tested.

Recommended Dilution:
Immunofluorescence: 10ug/ml
Immunoprecipitation/Western Blot: 4ug immunoprecipitates FAK from 500ug of a mouse 3T3/A31 cell RIPA lysate. The immunoprecipitate was resolved by electrophoresis, transferred to nitrocellulose and probed with F0019-56 (2ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.T.43Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceChickenMouse
Concentration:
~0.5mg/ml
Immunogen:
Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing p125 FAK.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as liquid in PBS, pH 7.2, 0.05% sodium azide, 30% glycerol.
Specificity:
Recognizes (and is specific for) chicken p125 FAK. Antibody has different affinity for modified forms of FAK and alternate splicing transcripts of FAK. Species Crossreactivity: human, rodent and avian.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kanner, S.B., et al., Proc. Natl. Acad. Sci. USA 87: 33283332, 1990. 2. Cobb, B.S., et al., Mol. Cell. Biol. 14: 147155, 1994. 3. Schaller, M.D., et al., Proc. Natl. Acad. Sci. USA 89: 51925196, 1992. 4. Carlson, T ., et al., J. Biol. Chem 276: 26516-26525, 2001. 5. Fernandez-Rodrigurez, et al., J. Biol. Chem. 273: 19321, 1998