Technical Data
F0019-75H
Fatty Acid Binding Protein, Heart (FABP)
Description:
The human Fatty Acid Binding Protein is derived from the human FABP3 gene. FABPs are small intracellular proteins (~13-14kD) with a high degree of tissue specificity that bind long chain fatty acids. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. H-FABP is localized in the heart, skeletal and smooth muscle, mammary epithelial cells, aorta, distal tubules of the kidney, lung, brain, placenta, and ovary. It is also useful as marker for brain damage.

Applications:
Suitable for use in ELISA, Immunoprecipitation, Western Blot and Purification. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:50 using reduced conditions. Detected a band of ~15kD.
Optimal dilutions to be determined by the researcher.

Positive Control:
Heart cells, recombinant human FABP

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16A546Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purified human Fatty Acid Binding Protein, Heart (FABP)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes human heart FABP of both natural and recombinant origin. Does not bind to human intestinal or liver FABP. Can be used to detect ischemia areas of human heart. Species Crossreactivity: rat, mouse and porcine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Roos, W et al; Monoclonal antibodies to human heart fatty acid-binding protein. J Immunol Meth 1995, 183: 149. 2. Guillame, E et al, Proteomics 3:1495 (2003). 3. Zimmermann-Ivol, C et al; Fatty acid binding protein as a serum marker for the early diagnosis of stroke. Mol Cell Proteomics 2004, 3: 66. 4. Pelsers, M et al; Brain- and heart-type fatty acid-binding proteins in the brain: tissue distribution and clinical utility. Clin Chem 2004, 50: 1568. 5. Zhen, E et al; Quantification of heart fatty acid binding protein as a biomarker for drug-induced cardiac and musculoskeletal necroses. Proteomics Clin Appl 2007, 1: 661.