Technical Data
F0024-01F
FbxL12 (F-box and Leucine-rich Repeat Protein 12, FBL12, FLJ20188)
Description:
FBXL12 (F Box and Leucine Rich Repeat Protein 12, FBXL 12, FLJ20188) is directed against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognize native protein in any application. May link a target protein and a ubiquitin-conjugating enzyme; contains an F-box domain.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16,000
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanGoat
Concentration:
0.25mg/ml
Immunogen:
Synthetic peptide corresponding to aa 315-326, [RACPKESMDWWM]of the C-Terminus of human FbxL12 protein. (NP_060173)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human FbxL12. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Winston JT et al. A family of mammalian F-box proteins. Curr Biol 9:1180-2 (1999).