Technical Data
FBXW2 (F-box and WD Repeat Domain Containing 2, F-box and WD40 Domain Protein 2, FBW 2, FBW2)
F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats.

Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:128,000
Western Blot: 0.3-1.5ug/ml; ~48kD band observed in Human Kidney lysates (calculated MW of 48.0kD according to NP_036296.1). A stronger band of unknown identity was also consistently observed at appr. 30kD - both bands were successfully blocked by incubation with the immunising peptide.
Immunohistochemistry: 2-3ug/ml In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to C-KRGSSFLAGEHPG, from the C Terminus of human FBXW2 (NP_036296.1).
Purified by peptide affinity chromatography.
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Recognizes human FBXW2. Species sequence homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW. A family of mammalian F-box proteins.Curr Biol. 1999 Oct 21;9(20):1180-2.