Technical Data
F4100-14E
a-Fetoprotein (AFP, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP )
Description:
Alpha fetoprotein receptor (AFP receptor) is a 62-67kD cell surface protein that mediates the uptake of AFP by proliferating or differentiating cells. AFP is expressed during gestation, but after birth levels in healthy adults drop to trace amounts. High expression of AFP and AFP receptor in adults can be indicative of certain cancers, especially hepatocellular carcinoma.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Paraffin): Does not require protein digestion pre-treatment of paraffin sections. Does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a9A166Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human alpha fetoprotein receptor.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide.
Specificity:
Recognizes human alpha fetoprotein receptor (AFP receptor).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Li, M. S. et al (2002) The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3T3 cells. Cell Res. 12:151-156. 2. Wang, X. W. and Xu, B. (1998) Stimulation of tumor-cell growth by alpha-fetoprotein. Int. J. Cancer 9:596-599.