Technical Data
FIAF (Fasting-induced Adipose Factor, Angiopoietin-like Protein 4, ANGPTL 4, PPAR gamma Angiopoitein-related Protein, PGAR, Hepatic Fibrinogen Angiopoietin-related Protein, HFARP, Bk89, Ng27, PPARG, fibrinogen Angiopoietin Related protein, major histocomp
The fasting-induced adipose factor (FIAF, ANGPTL4, PGAR, HFARP) was previously identified as a novel adipocytokine that was up-regulated by fasting, by peroxisome proliferators- activated receptor agonists, and by hypoxia. FIAF and other adipocytokines including resistin, adiponectin, acylationstimulating protein, plasminogen activator inhibitor-1, rennin angiotensin system, metallothioneins and tumor growth factor-b have been implicated in a variety of different processes, ranging from blood pressure control to lipid metabolism and insulin senziting. It has been tempting to attribute many of the clinical abnormalities associated with obesity from insulin resistance to altered secretion of particular adipocytokines.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:32000
Western Blot: 0.5ug/ml. ~ 26kD in Mouse Liver and Rat Liver lysates after antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.5kD according to mouse NP_065606.1.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ug-20CBlue IceMouseGoat
As reported
Synthetic peptide of murine Angptl4.
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Recognizes mouse Angptl4. Species Crossreactivity: Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lichtenstein L, Berbe JF, van Dijk SJ, van Dijk KW, Bensadoun A, Kema IP, Voshol PJ, Mller M, Rensen PC, Kersten S. Angptl4 upregulates cholesterol synthesis in liver via inhibition of LPL- and HL-dependent hepatic cholesterol uptake. Arterioscler Thromb Vasc Biol. 2007 Nov;27(11):2420-7. Epub 2007 Aug 30.