Technical Data
F4195-03
Fibrillin 1
Description:
Fibrillin 1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. Fibrillin 1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in Fibrillin 2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils.

Cellular Localization: Extracellular microfibrils.

Positive Control: Normal skin fibroblasts.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (Fr). Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k8k192Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceBovineMouse
Concentration:
~0.2mg/ml
Immunogen:
Microfibrils from zonular apparatus of bovine eye. Epitope: Amino acids 451 - 909.
Purity:
Purified by Protein G affinity chromatography.
Form
10mM PBS, pH7.4, 0.2%BSA
Specificity:
Species Crossreactivity: Crossreacts with Human and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Maier A et al. Fibrillin and elastin networks in extrafusal tissue and muscle spindles of bovine extraocular muscles. Invest Ophthalmol Vis Sci 35:3103-10 (1994). Wright DW et al. Immunization with undenatured bovine zonular fibrils results in monoclonal antibodies to fibrillin. Matrix Biol 14:41-9 (1994).