		Technical Data
F4195-03	Fibrillin 1

Description:
Fibrillin 1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. Fibrillin 1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in Fibrillin 2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils.

Cellular Localization: Extracellular microfibrils.

Positive Control: Normal skin fibroblasts.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (Fr). Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1,k	8k192	Affinity Purified

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Bovine	Mouse

Concentration:

~0.2mg/ml

Immunogen:

Microfibrils from zonular apparatus of bovine eye. Epitope: Amino acids 451 - 909.

Purity:

Purified by Protein G affinity chromatography.

Form

10mM PBS, pH7.4, 0.2%BSA

Specificity:

Species Crossreactivity: Crossreacts with Human and bovine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Maier A et al. Fibrillin and elastin networks in extrafusal tissue and muscle spindles of bovine extraocular muscles. Invest Ophthalmol Vis Sci 35:3103-10 (1994). Wright DW et al. Immunization with undenatured bovine zonular fibrils results in monoclonal antibodies to fibrillin. Matrix Biol 14:41-9 (1994).