Technical Data
F4203-02F2
Fibrinogen (Coagulation Factor I) (HRP)
Description:
Fibrinogen is a 340kD soluble plasma glycoprotein which is synthesized by the liver. Fibrinogen is an inactive zymogen which is activated by thrombin in the coagulation cascade to form fibrin, the most abundant component of blood clots .Fibrinogen activation is also involved in cellular and matrix interactions, the inflammatory response, cardiovascular disease and neoplasia.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:5-1:50
ELISA: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanSheep
Concentration:
~1mg/ml
Immunogen:
Native Fibrinogen
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.01% thimeorsal. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes human fibrinogen. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Brill, A. et al. (2011) von Willebrand factor-mediated platelet adhesion is critical for deep vein thrombosis in mouse models. Blood. 117: 1400-7. 2. Barrera, V. et al. (2011) Host fibrinogen stably bound to hemozoin rapidly activates monocytes via TLR-4 and CD11b/CD18-integrin: a new paradigm of hemozoin action. Blood. 117: 5674-82. 3. Grainger, D.J. et al. (2004) Apolipoprotein E modulates clearance of apoptotic bodies in vitro and in vivo, resulting in a systemic proinflammatory state in apolipoprotein E-deficient mice. J Immunol. 173: 6366-75. 4. Grainger, D.J. et al. (2001) Suppressing Thrombin Generation is Compatible With the Development of Atherosclerosis in Mice Thromb Res. 102: 71-80. 5. Chien, H.W. (2013) Surface conjugation of zwitterionic polymers to inhibit cell adhesion and protein adsorption. Colloids Surf B Biointerfaces. 107: 152-9. 6. Plskova, J. et al. (2004) Quantitative evaluation of the corneal endothelium in the mouse after grafting. Br J Ophthalmol. 88: 1209-16. 7. Kamath, S. and Lip, G.Y. (2003) Fibrinogen: biochemistry, epidemiology and determinants. Q.J.M. 96: 711-729.8. Mosesson, M.W. (2005) Fibrinogen and fibrin structure and functions. J. Thromb. Haemost. 3: 1894-1904.