Technical Data
Fibroblast Growth Factor, Acidic, Recombinant, Human (FGF Acidic, FGFa, aFGF, FGF alpha, Fibroblast Growth Factor 1, FGF1, AFGF, Beta Endothelial Cell Growth Factor alpha, ECGFA, Endothelial Cell Growth Factor beta, ECGF-beta, ECGFB, ECGF, GLIO703, Hepari
Molecular Biology Storage: -20CShipping: Blue Ice
Human FGF acidic, also known as FGF 1 (fibroblast growth factor 1), is a member of the heparin-binding growth factor family, widely distributed in the peripheral and central nervous systems.

FGF 1 acts as a potent neurotrophic factor, playing a key role during prenatal development and postnatal growth/regeneration and is dependent upon interactions with heparin sulfate (HS) proteoglycans located on cell surfaces and in the extracellular matrix, and the four high affinity tyrosine kinase receptors, designated FGFR-1,-2,-3 and -4.

Bioactivity was determined by the dose-dependent stimulation of thymidine uptake by FGF receptor-expressing BaF3 cells. The ED50 is <10ng/ml.

Recombinant corresponding to human FGF acidic, expressed in E. coli.

Molecular Weight:
~15.8 kD (140aa Sequence)

1 x 105 units/mg

Endotoxin: 0.1 ng/ug

Suitable for use in Functional Assays. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:

Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with 50ul of 5mM sodium phosphate, pH 8.0. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human
Purity: Purified: ~95% (SDS PAGE, HPLC)
Form: Supplied as a lyophilized powder. Preservative free. Reconstitute with 50ul of 5mM sodium phosphate, pH 8.0.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.