Technical Data
Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant corresponding to 140aa from human Fibroblast Growth Factor expressed in E. coli.

Molecular Weight:

ED50 <10 ng/ml ( > 1 x 105 units/mg). Biological activity is determined by measuring the FGF-a dose dependent stimulation of 3H-thymidine incorporation into BaF3 cell expressing FGF receptors. For most in vitro applications, a concentration range of 1.0-50 ng/ml is recommended. FGF-a is a heparin-binding protein which stimulates the proliferation of mesenchymal cells, neuroectodermal cells and endothelial cells.

Endotoxin: 0.1ng/ug

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with Tris, pH 7.4, BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~95% (SDS-PAGE, HPLC)
Concentration: Not determined
Form: Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with 5mM Tris, pH 7.4, 0.1% BSA to 0.25-0.5 mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Subramony, P. and S.E. Dryer (1997) Neuregulins stimulate the functional expression of Ca2+-activated K+ channels in developing chicken parasympathetic neurons. Proc. Natl. Acad. Sci. USA 94:5934-5938.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.