Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)
|Molecular Biology||Storage: -20°CShipping: Blue Ice|
Single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. It binds to heparin, which potentiates its biological activity and protects it from proteolysis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages, and also has chemotactic and mitogenic activities. It was originally named acidic fibroblast growth factor based upon its chemical properties and to distinguish it from basic fibroblast growth factor.
Other homologous FGF belonging to the same family are int-2 (FGF-3 ), FGF-5 , FGF-6 , K-FGF and KGF (keratinocyte growth factor =FGF-7 ). All factors are products of different genes, some of which are Oncogene products (FGF-3 , FGF-4 , FGF-5 ).
Recombinant Human FGF-a (FGF-1) produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of 15803 Dalton.
Recombinant Human FGF-a is purified by proprietary chromatographic techniques.
Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Asn-Leu-Pro.
Dimers and aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.
Recombinant Human FGF-a is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <10ng/ml, corresponding to a specific activity of 105Units/mg.
0.1ng/ug (IEU/ug) of FGF-acidic-Sf9.
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of FGF-a as a Reference Standard.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Sf9 insect cells
Purity: 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form: Supplied as a liquid in 20mM Tris HCl pH 7.9, 100mM KCl, 0.2mM DTT and 20% glycerol.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.